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psb tet neo vector  (Addgene inc)


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    Addgene inc psb tet neo vector
    Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – <t>pSB</t> <t>tet</t> <t>-Neo</t> <t>vector</t> containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).
    Psb Tet Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psb tet neo vector/product/Addgene inc
    Average 92 stars, based on 10 article reviews
    psb tet neo vector - by Bioz Stars, 2026-04
    92/100 stars

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    1) Product Images from "Neuronal cell line expressing full-length mutant huntingtin exhibits alterations in proteolysis"

    Article Title: Neuronal cell line expressing full-length mutant huntingtin exhibits alterations in proteolysis

    Journal: bioRxiv

    doi: 10.64898/2026.01.15.699723

    Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – pSB tet -Neo vector containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).
    Figure Legend Snippet: Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – pSB tet -Neo vector containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).

    Techniques Used: Mutagenesis, Transgenic Assay, Expressing, Biomarker Discovery, Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection, Reverse Transcription, Western Blot, Control, Passaging



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    psb tet neo vector  (Addgene inc)
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    Addgene inc psb tet neo vector
    Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – <t>pSB</t> <t>tet</t> <t>-Neo</t> <t>vector</t> containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).
    Psb Tet Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psb tet neo vector/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    psb tet neo vector - by Bioz Stars, 2026-04
    92/100 stars
    		  Buy from Supplier

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    Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – pSB tet -Neo vector containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).

    Journal: bioRxiv

    Article Title: Neuronal cell line expressing full-length mutant huntingtin exhibits alterations in proteolysis

    doi: 10.64898/2026.01.15.699723

    Figure Lengend Snippet: Application of the Sleeping Beauty system for the transposition of normal/mutant huntingtin genes into the genome of Neuro-2a cells allows to create HD transgenic cellular model and determine the levels of HttQ15/Q138 genes expression in obtained cell lines. a) The validation of transgenes expression by quantitative PCR (qPCR). SB::Q15/Q138 – pSB tet -Neo vector containing HttQ15 or HttQ138 gene, SB::(-) – pSB tet -Neo vector without huntingtin gene insertion, -SB – Neuro-2a cells without transfection, RT – reverse transcription; b) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression; c) Western blotting of poly/monoclonal transgenic Neuro-2a cells lysates without/with doxycycline induction of HttQ15 or HttQ138 expression. Data for control Neuro-2a lines (SB::(-), with transfection of «empty» vector, and -SB, without transfection, are represented also. Top panel – lysates of four different monoclonal transgenic Neuro-2a lines with HttQ15 expression, middle panel – same as top, but for lysates of HttQ138 monoclonal lines, bottom panel – lysates of polyclonal transgenic Neuro-2a lines; d) The transgene (HttQ15/HttQ138) copy number in the genomes of monoclonal (Q15m/Q138m) and polyclonal (Q15p/Q138p) Neuro-2a lines. The GAPDH-normalized Htt gene copy number in the SB::(-) control line was referred to 1. e) 2 -ΔΔCt values for transgenic Neuro-2a lines with HttQ15 or HttQ138 expression, (f) Western blotting of lysates without/with doxycycline induction of HttQ15 or HttQ138 expression after 3 years of storage, cultivation and passaging. Total protein content in each sample was controlled by β-actin immunoreactivity. Data in Tables b) and f) and Fig. d) are represented as mean ± S.D. (n = 3).

    Article Snippet: The original pSB tet -Neo vector was from Addgene (#60509).

    Techniques: Mutagenesis, Transgenic Assay, Expressing, Biomarker Discovery, Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection, Reverse Transcription, Western Blot, Control, Passaging